HCM011 Selenite Cystine Broth

Usage:

For selective isolation of shigella and salmonella from clinical specimens and foods.

Approximative Formula (Per Liter):

Xylose 3.5g

L-Lysine 5g

Lactose Monohydrate 7.5g

Sucrose 7.5g

Sodium Chloride 5g

Yeast Extract 3g

Phenol red 80mg

Agar 13.5g

Sodium Deoxycholate 2.5g

Sodium thiosulfate 6.8g

Ferric Ammonium Citrate 0.8g

Final pH 7.4±02

Directions:

Suspend 55.2g in 1Lof distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Do not autoclave ,cool to 50°C and pour into sterile petri dishes.

Storage:

Keep container tightly closed, store in a dry place, away from bright light.